Effects of kinetin on L-phenylalanine ammonia-lyase activity in tobacco cell culture.
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چکیده
منابع مشابه
l-Phenylalanine Ammonia-Lyase Activity During Germination of Phaseolus vulgaris.
l-Phenylalanine ammonia-lyase (PAL) activity develops in excised bean axes after approximately 5 hours of incubation and reaches a maximum level after 14 hours of incubation. Light does not affect the development of activity, but puromycin, cycloheximide, actinomycin D, and 5-fluorouracil inhibit.During this period of incubation both d- and l-p-fluorophenylalanine stimulate fresh weight increas...
متن کاملYeast Phenylalanine Ammonia-lyase
I?henyialanine ammonia-lyase from the yeast Rhodotorula gluiinis was purified by salt fractionations and Sephadex chromatography. Density gradient centrifugation and Sephadex chromatography indicated its molecular weight to be about 275,000. Enzymatic deamination of several ring-substituted phenylalanine analogues and n-phenylalanine was studied. While cinnamic acid, a product of deamination, a...
متن کاملincreased podophyllotoxin production and phenylalanine ammonia lyase activity in cell culture of linum album by methyl jasmonate
linum album l. is containing pharmaceutical composition podophyllotoxin(ptox) which is currently being used as a precursor to semi-synthetic anticancer drugs etoposide, teniposide and etopophos. cell culture is as an important source for the production of ptox. several strategies, including treatment with elicitors have shown for enhanced production of secondary metabolites in cell culture syst...
متن کاملl-Phenylalanine Ammonia-lyase Activity in Robinia pseudoacacia Seedlings: I. Cyclic Phenomenon Activity during Continuous Light.
Influences of light and darkness on l-phenylalanine ammonia-lyase activity of black locust (Robinia pseudoacacia) seedlings were studied. Light initiates both an increase in enzyme activity (or enzyme synthesis) and a subsequent decrease in activity. These dual influences of light create cyclic changes in l-phenylalanine ammonia lyase activity in continuous light. The cyclic changes in activity...
متن کاملCloning and sequencing of a full-length cDNA coding for phenylalanine ammonia-lyase from tobacco cell culture.
PAL (EC 4.3.1.5) plays a key role in linking primary metabolism to phenylpropanoid metabolism by catalyzing the deamination of L-Phe to produce trans-cinnamic acid. This reaction is considered a key step in phenylpropanoid metabolism. In bean (Edwards et al., 1985) and parsley (Lois et al., 1989), PAL genes are transcriptionally activated by elicitor treatment, UV light, and wounding. We report...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1988
ISSN: 0002-1369,1881-1280
DOI: 10.1271/bbb1961.52.2617